PCR-based detection of Babesia bovis and Babesia bigemina in their natural host Boophilus microplus and cattle
نویسندگان
چکیده
منابع مشابه
PCR-based detection of Babesia bovis and Babesia bigemina in their natural host Boophilus microplus and cattle.
PCR and nested-PCR methods were used to assess the frequency of Babesia bovis and Babesia bigemina infection in Boophilus microplus engorged females and eggs and in cattle reared in an area with endemic babesiosis. Blood and the engorged female ticks were from 27 naturally infested calves and 25 crossbred cows. The frequency of both Babesia species was similar in calves and cows (P>0.05). Babes...
متن کاملMolecular Detection of Babesia bigemina and Babesia bovis in Crossbred Carrier Cattle Through PCR
Bovine babesiosis and other tick borne diseases are considered responsible for more than 50% losses in the crossbred cattle. Carrier cattle infected with babesiosis are challenge to current diagnostic methods and are difficult to detect because of the low number of parasites in circulation. However, diagnosis of carrier animals in herd is important for preventing outbreaks by transmission throu...
متن کاملExperimental Transmission of Babesia bigemina by Boophilus decoloratus in Cattle
Experimental transmission of B.bigemina between cattle and ticks Boophilus decoloratus were carried out in the laboratory. Body bags were applied on the bodies of two calves infected with B. bigemina.These were the four month old splenectomized male Friesian calf number 10B and the two month old intact male Ayrshire calf number 28B. B. decoloratus larvae were then introduced into these body bag...
متن کاملDevelopment of TaqMan-based real-time PCR assays for diagnostic detection of Babesia bovis and Babesia bigemina.
Two TaqMan-based real-time polymerase chain reaction (PCR) assays devised for the detection of two bovine Babesia parasites, Babesia bovis and B. bigemina, were evaluated for their diagnostic utility using cultured parasites and 92 field bovine blood samples collected from cattle living in Brazil. The real-time PCR assays were compared with previously established nested-PCR assays. The detectio...
متن کاملDetection of Babesia bovis in cattle by PCR-ELISA.
We established a new highly sensitive method, PCR-ELISA, for the dectection of Babesia bovis in cattle for farms in Thailand. The detection of around 2.4 x 10(-8)% parasitemia (equivalent to 1 infected erythrocyte per 2 ml) was achieved by PCR amplification followed by the ELISA detection of a biotin tagged gene. When comparing the sensitivity of PCR-ELISA with the microscopic method, our PCR-E...
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ژورنال
عنوان ژورنال: International Journal for Parasitology
سال: 2005
ISSN: 0020-7519
DOI: 10.1016/j.ijpara.2004.09.002